Quantification western blot avec image j manual

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A digital image of the band signal and intrinsic background for calculating protein band volume on. With western blot bands, the "base" is the area where theI have quite a number of western blots to be quantified. I am also looking for alternative techniques to quantify protein bands. ImageJ. Binary images were then converted to outlines using ImageJ. The western blots were captured with a digital camera, and the intensities were quantified There are two built in methods for analyzing a dot blot in ImageJ. The first is to treat each row as a horizontal "lane" and use ImageJ's gel analysis function. Densitometry using ImageJ Convert the image to 8-bit using ImageJ function (Image→Type→8-bit). A window with lane plots will appear.