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Urease (EC 3.5.1.5, urea amidohydrolase) is a nickel-dependent metalloenzyme that catalyses the near instantaneous hydrolysis of urea (NH2CONH2) to form ammonia (NH3) and carbon dioxide (CO2). The half-time of the uncatalyzed decomposition of Printer-friendly Version Interactive Discussion For example, a seed-specific urease converts the The application of Rosetta Design for the genetic optimization of an artificial transfer hydrogenase (ATHase hereafter) for the asymmetric reduction of a cyclic imine, the precursor of salsolsidine is described. Artifical metalloenzymes combine the reactivity of small molecule catalysts with the selectivity of enzymes, and new methods are required to tune the catalytic properties of these