Indole test procedure pdf

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Upon completion of this exercise, a student should be able to: • Understand the underlying mechanism of the indole test;. • Properly conduct the indole test;The indole test determines the ability of an organism to produce indole from the breakdown of the amino acid tryptophan. Tryptophan is hydrolyzed by SUMMARY AND EXPLANATION. Previous tests for the detection of indole production required a xylene extraction procedure to detect small amounts of indole