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The Cq and relative expression level are calculated by the Biorad CFX Manager 3.1 based on the MIQE guidelines (Bustin et al., 2009). The following are trademarks of Bio-RadLaboratories, Inc.: Bio-Rad, CFX Maestro, CFX384, CFX384 Touch,. CFX96 Touch, CFX Connect, and C1000 Touch and iProof Bio-Rad qPCR (quantitative PCR) Real Time Gradient Thermal Cycler C1000 and CFX Manager Software 3.1 is included (CD Copy only / no licence required) CFX96 Touch. ™. , CFX96 Touch. Deep Well. ™. , CFX Connect. ™. , and. CFX384 Touch. ™. Real-Time PCR. Detection Systems. Instruction Manual. Information contained in this manual is copyrighted by Bio-Rad Laboratories, 3.1 Overview . 3.8.1 CFX Manager Software Installation . CFX Manager 3.1 software (Bio-Rad, 2012) was used to evaluate the relative gene expression. Inter-run calibrations were used for each panel of experimentsMiniOpticon System Instruction Manual For MiniOpticon real-time PCR detection system with CFX Manager software Catalog #CFB Bio-Rad Laboratories, CFX Manager software automatically subtracts the baseline from well data. Select Settings from the menu bar, and then select Baseline Threshold to change the CFX96™ Deep Well Dx System (IVD, Bio-Rad), part of DT16 Revision der Software: 3.1 Manual de operação Sistemas CFX96™ Dx e CFX96 Deep Well Dx. Bio-Rad Laboratories, Inc. periodically offers updates for C1000 Touch thermal cycler, CFX96 Touch Touch system firmware and for CFX Manager™ software. and CFX Manager™ software CD are required for installation. Bio-Rad CFX, then open and follow instructions in the Readme.txt file. contact your local Bio-Rad office. • MiniOpticon system. • USB cable. • CFX ManagerTM software installation CD. • Instruction manual. • CFX Manager software contact your local Bio-Rad office. • MiniOpticon system. • USB cable. • CFX ManagerTM software installation CD. • Instruction manual. • CFX Manager software on the CFX96 machine next to the computer. • Double-click on the icon labeled Bio-. Rad CFX Manager. • Login to the software. If you do not have a user ID. We have 1 Instruction Manual and User Guide for CFX Manager™ Software Bio-Rad. Bio-Rad CFX Manager™ Software User Manual, 178 pages. Follow the instructions on-screen to complete installation. When completed, the Bio-Rad. CFX manager software icon will appear on the desktop of the computer. 5